Stoff	2,4,6-Trinitrotoluol
Literaturtitel	Chronic toxicity of 2,4,6-trinitrotoluene to a marine polychaete and an estuarine amphipod
Organismus	Leptocheirus plumulosus
Habitat	Sediment
Prüfart	Mortalität
Endpunkt	Überlebende
Standard
Dauer Parameter Wert Bezug nominal / analytisch 28  d LC50 = 202,7  mg/kg TG Dauer (norm.) Wert (norm.) 28  d LC50 = 202,7  mg/kg TG
Testmedium	Sediment
pH	7,8	Temperatur	21,3 °C
Bodenart		KAK
Corg [%]	2,66	Ton [%]
Methodenlisting
Durchführung test chambers (250 ml glass beakers). 25 ml of test sediment (1 cm) and 175 ml of 5 ppt artificial seawater. 6 replicates per treatment Futter Tetramin 3x weekly (1.5 mg/animal, 1.-2. w, then 3 mg/animal 3. w) Konzentrationsbestimmung measured concentrations reported as TNT represent TNT and its degradation products Konzentrationen seven treatments, including one methanol-carrier control Matrixbezug Sediment Organismus Alter/Lebenstadium juvenile (7–14 d) Organismus Herkunft laboratory cultures (original stock, 1992, James Pond, Magothy River, MD, USA) Organismus Anzahl 10 amphipods per replicate Testsubstanz Uniformly ring-labeled [14C] TNT, New England Nuclear Research Products (Boston, MA, USA), specific activity of 23.6 mCi/mmol and Nonlabeled TNT (Sigma Chemical Company, St. Louis, MO, USA) Testsubstanz, Reinheit [14C] TNT (99%), TNT (97%) Lösevermittler Methanol Photoperiode 16:8 light:dark Testmedium clean sediment (Sequim Bay, WA, USA; presieved < 300 µm) und Meerwasser, künstlich Lösungsmittelkontrolle, Ergebnis Yes Salzgehalt 5 ppt