Stoff	2-Amino-4,6-dinitrotoluol
Literaturtitel	The role of metabolism in the toxicity of 2,4,6-trinitrotoluene and its degradation products to the aquatic amphipod Hyalella azteca
Organismus	Hyalella azteca
Habitat	Wasser
Prüfart	Mortalität
Endpunkt	Mortalität, Verhalten
Standard
Dauer Parameter Wert Bezug nominal / analytisch 96  h LC50 = 3,83  mg/l ana Dauer (norm.) Wert (norm.) 4  d LC50 = 3.830  µg/l ana
Testmedium	Leitungswasser
pH		Temperatur	23 ± 2 °C
Dynamik	semistatisch	Wasserhärte
Methodenlisting
Auswertung (Statistische Methode) Results from the toxicological effects experiments were evaluated by nonlinear regression of data using Prisms statistical software (Graphpad, San Diego, California, USA). The four-parameter logistic equation (Response ¼ Minimum+(MaximumMinimum)/(1+10^)((Log LC50-X) Hill Slope)) was used to calculate the LC50 values with 95 percent confidence intervals. Confidence intervals were used to indicate statistical differences where intervals did not overlap Konzentrationen 5.0–202.8 mM; 1.0–40 mg/L Organismus Alter/Lebenstadium Juvenile organisms collected were estimated to be 10–12 days of age based on a length of 2.170.2 mm (USEPA, 2000) Organismus Herkunft H. azteca, were cultured at the Engineering Research and Development Center, Vicksburg, Missisippi, USA with organisms originally obtained from the USGS Biological Resources Division, Environmental and Contaminants Research Center (Columbia, Missouri, USA) Testsubstanz, Reinheit >99% pure Analytik der Testsubstanz Chemical analysis for TNT and its degradation products in water was conducted using an Agilent 1100 HPLC (Palo Alto, California, USA) equipped with a diode-array detector using a modification of US EPA method 8330 (USEPA, 1997). Method detection limits for the analysis of water samples using this method ranged from 0.003 to 0.01 mg/L