Stoff	2,4,6-Trinitrotoluol
Literaturtitel	Toxicity and fate of two munitions constituents in spiked sediment exposures with the marine amphipod Eohausorius estuaries
Organismus	Eohaustorius estuarius
Habitat	Sediment
Prüfart	Mortalität
Endpunkt
Standard	EPA 600/R-94/025
Dauer Parameter Wert Bezug nominal / analytisch 10  d LC50 = 23  mg/kg TG real Dauer (norm.) Wert (norm.) 10  d LC50 = 23  mg/kg TG real
Testmedium	Sediment
pH	7,6 - 8,2	Temperatur	14,8 - 16,1 °C
Bodenart		KAK
Corg [%]	0,36	Ton [%]	23
Methodenlisting
Durchführung 1-L glass beakers containing 200 g of spiked sediment and 800 ml of 20‰ synthetic seawater. Five replicates per test concentration. Auswertung (Statistische Methode) trimmed Spearman-Karber Method Futter without feeding Konzentrationsbestimmung sum-molar concentrations of 2,4,6-trinitrotoluene (TNT) and TNT degradation products = TNT* Organismus Alter/Lebenstadium 3 - 5 mm Organismus Herkunft clean field site, acclimated slowly to the 20‰ experimental salinity Testsubstanz Radiolabeled trinitrotoluene ([14C]TNT, 23.6 Ci/mol) New England Nuclear Research Products and Nonradiolabeled TNT (99% pure) Chem Service Sauerstoffgehalt 8,1 - 9,8 mg/l Testsubstanz, Reinheit radiochemical purity (99%) and nonradiolabeled TNT (99%) Analytik der Testsubstanz Yes. liquid scintillation counting (LSC), and high-performance liquid chromatography (HPLC) Lösevermittler acetone Konzentrationen, nominal 25 | 50 | 100 | 200 | 400 | mg/kg dry wt (sediment SDB) Kontrollen (negative) survival 82 +/- 8% Testmedium Uncontaminated surficial sediments, (total solids content 5 67%; silt and clay content 5 23%; total organic carbon content 5 0.36%), San Diego Bay (SDB), California, USA. Collection-site sediment (YB) was used as the negative control. Lösungsmittelkontrolle, Ergebnis nr Salzgehalt 20 g/l
Memos
Bemerkung	LC50 = 125 µmol/kg dry wt
Testbonität
RI-3 / CRED
Ableitung	Nein. Da ein straker und nicht linearer Abbau von TNT in den Testansätzen auftrat.