Stoff	2,4,6-Trinitrotoluol
Literaturtitel	TOXICITY OF NITROGUANIDINE, NITROGLYCERIN,HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE(RDX), AND 2,4,6-TRINITROTOLUENE (TNT) TO SELECTED FRESHWATER AQUATIC ORGANISMS
Organismus	Lemna minor
Habitat	Wasser
Prüfart	Wachstumshemmung
Endpunkt	Wachstumsrate
Standard
Dauer Parameter Wert Bezug nominal / analytisch 96  h LOEC = 1,21  mg/l real Dauer (norm.) Wert (norm.) 4  d LOEC = 1.210  µg/l real
Testmedium	Süßwasser, künstlich
pH	7,7 (7,3 - 8,1)	Temperatur	25 °C
Dynamik	semistatisch	Wasserhärte	35 (14-46)
Methodenlisting
Auswertung (Statistische Methode) using the inhibition proportion" technique recommended by Horning and Weber (1985). The technique uses the probit analysis to estimate EC50s and their 95% fiducial limits. Since the assumptions of the probit analysis are not met in the classical sense because of the very nature of the growth data, the count data at each treatment were averaged and subsequently converted to "inhibition proportions" using the formula below before the probit analysis was performed. I = C - T / C * 100 where: C = the mean growth of the controls T = the mean growth at a given treatment In addition to the EC50s for growth, the no-observed-effect concentrations (NOEC) and lowest-observed-effect concentrations (OEC) were determined by Dunnett's test. Dunnett's test consists of an analysis of variance (ANOVA) to determine the error term, which is then used in a multiple comparison method for comparing each of the treatment means with the control mean. The assumptions upon which the use of Dunnett's test is contingent are that the observations within treatments are independent and normally distributed, with homogeneity of variance. The chi-square test for normality and Bartlett's test for homogeneity of variances were performed before the Dunnett's test was used. The above statistical tests were perform using Toxatat (Gulley at al., 1989). Organismus Herkunft obtained from a local pond Analytik der Testsubstanz A Waters HPLC system (Waters Associates, Milford, MA) was used. Column: Waters µBondapak C18 Mobile Phase: 55% methanol:45% water Method: Isocratic Flow Rate: 1.0 mL/min 'Detector: UV 240 na, 0.1 AUFS Inj&ection Volume: 10-200 I&L depending on the concentration of material in solution Testsystem, Multispezies 10 two-frond colonies per replicate were exposed to each treatment in 250 mL glass beakers containing 150 mL nutrient medium described in Wang (1986). Triplicates were run for each treatment and control. All test solutions were renewed at 24-h intervals. Observations on frond production were made at each renewal period and at the end of the test Konzentrationen, nominal 0,3; 0,6; 1,25; 2,5; 5 [mg/l] Konzentrationen, real 0,28; 0,59; 1,21; 2,43; 4,92 [mg/l] Lichtstärke The colonies were maintained under constant cool-white fluorescent lights (300 foot candles)