Stoff	2,4,6-Trinitrotoluol
Literaturtitel	Toxicity of Explosive Compounds to the Marine Mussel, Mytilus galloprovincialis, in Aqueous Exposures
Organismus	Mytilus galloprovincialis
Habitat	Wasser
Prüfart	Mortalität
Endpunkt	Überlebende
Standard	ASTM E 724-98 (1999)
Dauer Parameter Wert Bezug nominal / analytisch 96  h LC50 = 19,5  mg/l real Dauer (norm.) Wert (norm.) 4  d LC50 = 19.500  µg/l real
Testmedium	Meerwasser, künstlich
pH	7,4 - 8,1	Temperatur	15 °C
Dynamik	semistatisch	Wasserhärte
Methodenlisting
Auswertung (Statistische Methode) Maximum likelihood-probit or trimmed Spearman–Karber analyses. ToxCalc software (Version 5.0, Tidepool Scientific, McKinleyville, CA, USA). Futter not fed during the exposure Organismus Alter/Lebenstadium Small adult mussels with a mean length of 3.95 (+/-0.25) cm and wet tissue mass of 1.97 (+/-0.45) g were used for the adult exposures. Larger adult mussels (7–10 cm) from the same source were used to obtain gametes for the embryo–larval development toxicity tests. Organismus Herkunft Carlsbad Aquafarm (Carlsbad, CA, USA) Endpunktbestimmung Lethality was determined by presence of open valves (relaxed adductor muscles) that were unresponsive to stimuli. Sauerstoffgehalt 6.4 - 9.3 mg/L Analytik der Testsubstanz HPLC, laboratory reporting limit 0.1 mg/L Lösevermittler <= 1mL/L for any test dilution, and acetone controls (1mL/L1) Testsystem, Multispezies 4 replicates 1-L glass beakers for each test concentration Konzentrationen, nominal 0 | 0,38 | 1,5 | 3 | 10 | 30 | mg/l Konzentrationen, real ND | 0,26 | 0,56 | 1,24 | 2,61 | 8,55 | 28,1 | mg/l Auswertung (95%-CL) LC50 19.5 (17.0–27.2) mg/L Lichtstärke yellow fluorescent light (Lambda > 500 nm) to minimize photodegradation Testmedium 30 ppt synthetic seawater (Instant Oceans, Aquarium Systems, Inc., Mentor, OH, USA) Lösungsmittelkontrolle, Ergebnis No mortality was observed in either the solvent or clean seawater negative controls.