Stoff	2,4,6-Trinitrotoluol
Literaturtitel	Toxicity of TNT Wastewaters to Aquatic Organisms. Volume 1. Acute Toxicity of LAP Wastewater and 2,4,6-Trinitrotoluene
Organismus	Pimephales promelas
Habitat	Wasser
Prüfart	Mortalität
Endpunkt	Verstorbene
Standard
Dauer Parameter Wert Bezug nominal / analytisch 96  h LC50 = 2,9  mg/l ana Dauer (norm.) Wert (norm.) 4  d LC50 = 2.900  µg/l ana
Testmedium	Leitungswasser
pH	7,7	Temperatur	20±1 °C
Dynamik	statisch	Wasserhärte	33,8
Methodenlisting
Auswertung (Statistische Methode) To estimate the median lethal concentration (LC50) we used a computerized program developed at SRI and composed of several statistical methods for estimating LC50s and EC50s. For this project, we chose' estimates derived only from the log-probit or binomial methods. The specific method we chose depended on the number and pattern of partial responses , (i.e., >0 percent, <100 percent). Organismus Herkunft SRI's breeding colonies Analytik der Testsubstanz HPLC. The instrument (Spectra-Physics, Model 3500B) was equipped with a 3.9 m by 30 cm C1 8 - Bondapak colunn for the analysis of TNT, RDX, TNT/RDX, and DNA. and with a 3.9 mm x 30 cm Spherisorb ODS column for the analysis of THB and TNBA. Various mixtures of methanol and water were used as eluting solvents. All analyses were perforu.ad by direct injection of the aqueous samples into the chromatograph. Table 5 presents the HPLC parameters for those toxicants. Methode nach Quelle "Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians" (EPA, 1975) was used as a guide in conducting the tests with the animal species