Stoff	2,4,6-Trinitrotoluol
Literaturtitel	TOXICITY OF NITROGUANIDINE, NITROGLYCERIN,HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE(RDX), AND 2,4,6-TRINITROTOLUENE (TNT) TO SELECTED FRESHWATER AQUATIC ORGANISMS
Organismus	Pimephales promelas
Habitat	Wasser
Prüfart	Mortalität
Endpunkt	Verstorbene
Standard	ASTM E 729-80
Dauer Parameter Wert Bezug nominal / analytisch 96  h LC50 = 2,66  mg/l real Dauer (norm.) Wert (norm.) 4  d LC50 = 2.660  µg/l real
Testmedium	Süßwasser, künstlich
pH	7,9	Temperatur	25±1 °C
Dynamik	durchfluss	Wasserhärte	164
Methodenlisting
Auswertung (Statistische Methode) All concentration data used in the statistical analyses were mean measured concentrations. The 96-h LC50s and their 95% fiducial limits were determined by the probit method. Both the probit LC50s and their 95% fiducial and the moving 3 average determined angle by LC50s an EPAand statistical their 95%program confidence (Stephan, limits 1978).When the data sets met the assumptions of normality and homogeneity of variance, a parametric statistic was used. Dunnett's test was used when the number of replicates was constant among treatments. Duncan's new multiple range test was also occasionally used. A t-test with Bonferroni adjustment of error rate was performed when the number of replicates was not constant among treatments. When a data set failed to meet the cssumptions of normality or homogeneity of variance, a nonparametric statistic was used. Steel's Many-One Rank test was performed when equal number of replicates were used and at least four replicates per treatment were employed in the test. The Kruskal-Wallis procedure was used to compare differences between sample means when less than four replicates per treatment were used. The wilcoxon Rank Sum test with Bonferroni adjustment was used when unequal number of replicates occurred. The statistical tests were performed using SAS (1979) and Toxetat (Gulley et al.* 1989). A minimum prcbability level of 0.05 was used for all tests. Organismus Alter/Lebenstadium Juvenile, 23 days at beginning of test Organismus Herkunft Fathead minnow (2._ l) embryos and juveniles were obtained from the JHU/APL culture maintained at 25 (± 1) "C in JHU/APL well water. The JHU/APL culture procedures were similar to those recommend by Peltier and Weber (1985). The JHU/APL culture was initiated with mature fathead minnows obtained from the U.S. EPA Environmental Monitoring and Support Laboratory - Cincinnati, Ohio Sauerstoffgehalt 8,0 mg/l (7,6-8,3) Testsubstanz, Reinheit 99% Analytik der Testsubstanz Aqueous concentrations of TNT were determined by the HPLC method of Brueggenan (1983). A Waters HPLC system (Waters Associates, Milford, MA) was used. Column: Waters IsBondapak CUI Mobile Phase: 55% methanol:45% water Method: Isocratic Flow Rate: 1.0 mL/min 'Detector: UV 240 na, 0.1 AUFS Injection Volume: 10-200 µL depending on the concentration of material in solution