Stoff	2,4,6-Trinitrotoluol
Literaturtitel	Toxicity and bioaccumulation of 2, 4, 6-trinitrotoluene in fathead minnow (Pimephales promelas)
Organismus	Pimephales promelas
Habitat	Wasser
Prüfart	Mortalität
Endpunkt	Verstorbene
Standard
Dauer Parameter Wert Bezug nominal / analytisch 4  d LC50 = 2,7  mg/l real Dauer (norm.) Wert (norm.) 4  d LC50 = 2.700  µg/l real
Testmedium	Leitungswasser
pH	6-9	Temperatur	23 ± 1 °C
Dynamik	statisch	Wasserhärte	60-160 mg/L
Methodenlisting
Organismus 5- to 7-d-old fathead minnow larvae (0.58 ± 0.37 mg, n = 3) Dauer 4 days Auswertung (Statistische Methode) Measured experimental values are expressed as the mean ± standard deviation. One-way analysis of variance was used to determine differences between means at a 0.05 level of significance. Tukey’s method was used for multiple comparisons. Survival data were arcsine-squareroot transformed and complied with the requirement of normality and equal variance. The LC50s and median lethal tissue residues (LR50s) and associated 95% confidence limits (CLs) were calculated with the trimmed Spearman–Karber method using ToxCalc version 5.0 of treatment and control means Futter Fish were fed 0.2 ml of concentrated, newly hatched brine shrimp (Artemia nauplii) per individual daily both before and every 48 h during the experiments Organismus Herkunft Aquatic Research Organisms (Hampton, NH, USA) Organismus Anzahl 10 fish per replicate Sauerstoffgehalt > 4 mg/L Testsubstanz, Reinheit > 98% Analytik der Testsubstanz Chemical analysis of water samples was conducted using an Agilent 1100 HPLC (Palo Alto, CA, USA) equipped with a diode-array detector. The column used was a Supelco RP-Amide C-16 (Supelco, Bellefonte, PA, USA) with a sample injection volume of 100 l and flow rate of 1 ml/ min. Solvent ratios were 45% water and 55% methanol, and ultraviolet absorbance was measured at 230 nm Testsystem, Multispezies Three replicate exposure chambers (300-ml, tall form beakers) were used for all treatments. Each chamber contained 250 ml of exposure solution and 10 fish Konzentrationen, nominal Exposure treatments were 0.4, 2.2, 8.8, 22.0, and 44.0 µmol/L (0.1, 0.5, 2, 5, and 10 mg/L, respectively)
Memos
Bemerkung	Test nach EPA-821-R-02-012
Testbonität
2 / UBA-F+E