Stoff	2,4,6-Trinitrotoluol
Literaturtitel	TOXICITY OF NITROGUANIDINE, NITROGLYCERIN,HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE(RDX), AND 2,4,6-TRINITROTOLUENE (TNT) TO SELECTED FRESHWATER AQUATIC ORGANISMS
Organismus	Ceriodaphnia dubia
Habitat	Wasser
Prüfart	Bewegungsfähigkeit
Endpunkt	Motilität
Standard	ASTM E 729-80
Dauer Parameter Wert Bezug nominal / analytisch 48  h LC50 = 4,03  mg/l real Dauer (norm.) Wert (norm.) 2  d LC50 = 4.030  µg/l real
Testmedium	Süßwasser, künstlich
pH	8,1 (8,0 - 8,2)	Temperatur	24,9 °C
Dynamik	semistatisch	Wasserhärte	167
Methodenlisting
Auswertung (Statistische Methode) The 48- and 96-h LC50s and their 95% fiducial limits were determined by the probit method. In all cases, the goodness of fit probability of the data to the probit model was >0.05. When the probit method could not be used because the goodness of fit probability was <0.05 or there were less than two concentrations at which the percent mortality was between 0 and 100, the moving aierage angle method was used. Both the probit LC50s and their 95% fiducial and the moving 3 average determined angle by LC50s an EPAand statistical their 95%program confidence (Stephan, limits 1978) Futter The diet consisted of a mixture of Cerophyl* (Cerophyl Laboratories, Inc., Kansas City, MO) and the green alga, a. ca~ricornutum, added to the daphnid culture to achieve final concentrations of 120 Ag Cerophyl*/mL and 6.7 x 1 0S f. iaaricornutum cells/mL Ceriodadaphnia were fed the diet up to the initiation of each test, at the beginning of the test and at the 24-h renewal period during the test Organismus Herkunft Center for Lake Superior Environmental Studies, University of Wisconsin - Superior Organismus Anzahl A minimum of 10 organisms was run in each replicat Testsubstanz, Reinheit 99% Konzentrationen, nominal 0,57; 0,95; 1,58; 2,64; 4,4 [mg/l] Konzentrationen, real 0,5; 0,92; 1,44; 2,54; 4,02 [mg/l]