Stoff	2-Amino-4,6-dinitrotoluol
Literaturtitel	Toxicity of trinitrotoluene to sheepshead minnows in water exposures
Organismus	Cyprinodon variegatus
Habitat	Wasser
Prüfart	Mortalität
Endpunkt
Standard
Dauer Parameter Wert Bezug nominal / analytisch 5  d LC50 = 8,6  mg/l ana Dauer (norm.) Wert (norm.) 5  d LC50 = 8.600  µg/l ana
Testmedium
pH		Temperatur	23 °C
Dynamik		Wasserhärte
Methodenlisting
Auswertung (Statistische Methode) Trimmed Spearman-Karber analysis was used to calculate the median lethal concentration (LC50) and median lethal residue (LR50) values using ToxCalc software (Version 5.0, Tidepool Scientific, McKinleyville, CA). For survival data, one-way analysis of variance (ANOVA) was used to determine differences between means, using a 0.05 level of significance. Survival data were transformed by arcsine square root prior to statistical analysis. Dunnett’s test was used to determine significant differences from the control for concentration or residue means using ToxCalc software. Futter Fish were fed Artemia salina nauplii daily according to USEPA (2002) Organismus Alter/Lebenstadium Exposures were conducted using 4-week-old juvenile fish(15–25 mg). Testsubstanz, Reinheit from SRI International (Menlo Park, CA). The manufacturer-reported purity was <98% for all compounds Analytik der Testsubstanz Aqueous samples and solvent extracts were separated and quantified by high performance liquid chromatography (HPLC) following the U.S. Environmental Protection Agency SW-486 Method 8330. Testsystem, Multispezies Five replicates were used per treatment. Each replicate consisted of ten fish exposed to aqueous TNT solutions in 600 ml glass beakers. Test beakers were held in a recirculating water bath system at 23 1C under yellow fluorescent light (l4500 nm) to minimize photodegradation of the explosives. Water quality (pH, temperature, dissolved oxygen, salinity) was measured at exposure initiation and termination. Gentle aeration using a bubbler was necessary to maintain adequate and constant oxygen concentration. Konzentrationen, nominal 2; 4; 8; 12; 20 [mg/L]