Stoff	4-Amino-2,6-dinitrotoluol
Literaturtitel	DEVELOPMENTAL TOXICITY OF p,p-DICHLORODIPHENYLTRICHLOROETHANE, 2,4,6-TRINITROTOLUENE, THEIR METABOLITES, AND BENZO[a]PYRENE IN XENOPUS LAEVIS EMBRYOS
Organismus	Xenopus laevis
Habitat	Wasser
Prüfart	Embryotoxizität
Endpunkt	Embryonalentwicklung
Standard
Dauer Parameter Wert Bezug nominal / analytisch 96  h EC50 = 85,8  µmol/l nom Dauer (norm.) Wert (norm.) 4  d EC50 = 85,8  µmol/l nom
Testmedium	Süßwasser, künstlich
pH	6.5 to 8.5	Temperatur
Dynamik	semistatisch	Wasserhärte
Methodenlisting
Auswertung (Statistische Methode) On the basis of mortality and malformation incidence at each test concentration, the 96-h LC50 and 96-h EC50 for malformation values were calculated with 95% confidence intervals by probit analysis [25]. These values were used for comparative analyses of lethality and teratogenicity in the structurally related compounds. Organismus Alter/Lebenstadium Dejellying of fertilized eggs and selection of normal-appearing embryos were performed according to the protocol of American Society for Testing and Materials (ASTM) [18]. Embryos at stages 8 to 11 (midblastula to early gastrula) were used to start each test. Staging of the embryos was based on the normal table by Niewkoop and Faber Organismus Herkunft Adult pairs of X. laevis were obtained from Shimizu Laboratory Supplies (Kyoto, Japan) Testsubstanz, Reinheit 2ADNT, and 4ADNT (99.5% grade; AccuStandard, New Haven, CT, USA). Konzentrationen, nominal 6.69, 13.4, 26.8, 53.5, 107and 214 µM